Titusville is a city in Brevard County, Florida, United States. The population was 40,670 at the 2000 census. According to the U.S. Census Bureau's 2004 estimates, the city had a population of 42,614. It is the county seat of Brevard County. Called "Space City, USA," Titusville is a popular resort area situated near the Kennedy Space Center and Canaveral National Seashore. Titusville is located at 28°35′28″N, 80°49′12″W (28.591210, -80.819911). Titusville sits on the Indian River in the Northern half of Brevard County, Florida.According to the United States Census Bureau, the city has a total area of 67.1 km² (25.9 mi²). 55.1 km² (21.3 mi²) of it is land and 12.0 km² (4.7 mi²) of it (17.95%) is water. Titusville is located on the Indian River lagoon, part of the Atlantic Intracoastal Waterway.

As of the census of 2000, there were 40,670 people, 17,200 households, and 11,094 families residing in the city. The population density was 738.6/km² (1,913.4/mi²). There were 19,178 housing units at an average density of 348.3/km² (902.3/mi²). The racial makeup of the city was 83.80% White, 12.64% African American, 0.39% Native American, 0.94% Asian, 0.04% Pacific Islander, 0.73% from other races, and 1.46% from two or more races. Hispanic or Latino of any race were 3.52% of the population.