Lewiston, in Androscoggin County, is the second-largest city in the U.S. state of Maine. As of the 2000 census, the city's population was 35,690. It is one of two principal cities of and included within the Lewiston-Auburn, Maine Metropolitan New England City and Town Area and the Lewiston-Auburn, Maine Metropolitan Statistical Area (which is part of the Portland-Lewiston-South Portland, Maine Combined Statistical Area).

A former industrial center, it is located in southwest Maine, at the falls of the Androscoggin River, across from Auburn. Lewiston and Auburn are often thought of as a single entity and referred to as Lewiston-Auburn, which is sometimes abbreviated as L-A. Lewiston is home to Bates College, the Basilica of Saints Peter and Paul, the Lewiston campus of the University of Southern Maine, and two significant regional general hospitals, Central Maine Medical Center and Saint Mary's Regional Medical Center.