The City and Borough of Juneau is a unified municipality located on the Gastineau Channel on the Alexander Archipelago in the U.S. state of Alaska, and is the state capital. The municipality unified in 1970 when the City of Juneau merged with the City of Douglas and the surrounding borough to form the current home rule municipality.

The city, Alaska's third-largest in terms of population (after Anchorage and Fairbanks), is nestled at the base of Mount Juneau and across the channel from Douglas Island. As of the 2000 census, the City and Borough had a population of 30,711. The U.S. Census Bureau's 2004 population estimate for the City and Borough was 31,118.